重组人TEK蛋白

产品详情

  • 产品名称
    重组人TEK蛋白
  • 蛋白种属
    人
  • 表达宿主
    人胚胎肾细胞（HEK293）
  • Unitprot ID
    Q59HG2
  • 克隆构建
    A DNA sequence encoding the extracellular domain (Met 1-Lys 745) of human Tie2 (NP_000450.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
  • 蛋白纯度
    > 90 % as determined by SDS-PAGE
  • 蛋白活性
    Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.
  • 分子量
    The recombinant human Tie2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and predicts a molecular mass of 108.5 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2/Fc monomer is approximately 125-135 kDa in SDS-PAGE under reducing conditions.
  • 蛋白别名
    VMCM1; HYK; CD202B; AA517024; TIE2; TIE-2; RP23-345A23.1; VMCM; STK1; TEK
  • 蛋白全名
    Angiopoietin-1 receptor
  • 蛋白描述
    TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
  • 发货形式
    Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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