- 载体名称:
- pNEB193
- 载体抗性:
- Ampicillin
- 载体长度:
- 2713 bp
- 载体类型:
- Cloning Vectors
- 复制子:
- ori
- 载体来源:
- New England Biolabs
- 拷贝数:
- High copy number
- 5'引物:
- M13 fwd
- 3'引物:
- M13 rev
pNEB193 载体载体图谱
pNEB193 载体载体序列
LOCUS pNEB193. 2713 bp DNA circular SYN 01-JAN-1980 DEFINITION pUC19 derivative with additional sites for the 8-cutters AscI, PmeI, and PacI. ACCESSION . VERSION . KEYWORDS pNEB193. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2713) AUTHORS New England Biolabs TITLE Direct Submission REFERENCE 2 (bases 1 to 2713) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2713 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 396..479 /label=MCS /note="MCS" /note="multiple cloning site" primer_bind complement(492..508) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(516..532) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(540..570) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(585..606) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(894..1482) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1656..2513) /label=AmpR /note="beta-lactamase" promoter complement(2514..2618) /label=AmpR promoter
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