- 载体名称:
- pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
- 载体抗性:
- Ampicillin
- 载体长度:
- 10074 bp
- 载体类型:
- Mammalian Expression, Lentiviral
- 复制子:
- ori
- 拷贝数:
- High Copy
- 启动子:
- SV40
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS 载体载体图谱
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS 载体载体序列
LOCUS pHRdSV40-scFv-GC 10074 bp DNA circular SYN 13-MAY-2021 DEFINITION The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64. ACCESSION . VERSION . KEYWORDS pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10074) AUTHORS Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD TITLE A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. JOURNAL Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PUBMED 25307933 REFERENCE 2 (bases 1 to 10074) TITLE Direct Submission REFERENCE 3 (bases 1 to 10074) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..10074 /mol_type="other DNA" /organism="synthetic DNA construct" promoter complement(119..137) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(427..446) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 780..802 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(840..858) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 926..1030 /label=AmpR promoter CDS 1031..1888 /label=AmpR /note="beta-lactamase" rep_origin 2062..2650 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2804..2821 /label=L4440 /note="L4440 vector, forward primer" promoter 2896..3225 /label=SV40 promoter /note="SV40 enhancer and early promoter" intron 4359..4424 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4554..4574 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 4999..5133 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" LTR 5302..5935 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 5982..6107 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6599..6832 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7017..7061 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 7210..7251 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 7346..7463 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7526..7736 /label=SV40 promoter /note="SV40 early promoter" rep_origin 7595..7730 /label=SV40 ori /note="SV40 origin of replication" regulatory 7805..7814 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 8552..8578 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" CDS 8669..9379 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Nager et al., 2011)" CDS 9413..9562 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 9593..9754 /codon_start=1 /product="B1 domain of Streptococcal protein G " /label=GB1 /note="effective as a solubilizing fusion partner (Cheng and Patel, 2004)" /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD ATKTFTVTE" CDS 9782..9832 /codon_start=1 /product="nuclear localization signal from the human T-cell leukemia virus type 1 (HTLV-1) Rex protein" /label=Rex NLS /translation="PKTRRRPRRSQRKRPPT"
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