- 载体名称:
- pmini35S-EGFP-CmR-ccdB
- 载体抗性:
- Kanamycin, Chloramphenicol
- 载体长度:
- 5643 bp
- 载体类型:
- Gene expression
- 复制子:
- pSa ori
- 宿主:
- Plants
- 启动子:
- lac UV5
pmini35S-EGFP-CmR-ccdB 载体载体图谱
pmini35S-EGFP-CmR-ccdB 载体载体序列
LOCUS 62056_16815 5643 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5643) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5643 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 534..556 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" primer_bind 571..587 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" intron 733..922 /label=cat1 intron /note="castor bean catalase intron, modified" CDS 1019..1726 /codon_start=1 /label=mgfp5 /note="GFP with folding enhancement mutations" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN FKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELY" promoter 1849..1879 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 1933..2589 /codon_start=1 /label=CmR /note="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 2934..3236 /codon_start=1 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" /translation="MQFRVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" terminator 3329..3581 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3609..3625) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 3635..3659 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(3750..4338) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4512..5324) /codon_start=1 /label=KanR /note="aminoglycoside phosphotransferase" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 5615..5643 /label=pSa ori /note="origin of replication from bacterial plasmid pSa"
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