下载GeneBank文件(.gb)

LOCUS       62056_16260       15315 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2517..5828)
                     /gene="ABL1"
                     /label=ABL1
                     /note="Tyrosine-protein kinase ABL1 from Homo sapiens. 
                     Accession#: P00519"
     promoter        complement(8616..8983)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     promoter        9000..9510
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             9529..10272
                     /label=Tet-On(R) 3G
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of 
                     doxycycline"
     promoter        10286..10615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10624..11220
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    11237..11825
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             12033..12666
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     promoter        complement(12843..12873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12888..12909)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13197..13782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13956..14813)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(14814..14918)
                     /label=AmpR promoter
     polyA_signal    14966..15100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"