下载GeneBank文件(.gb)

LOCUS       40924_13055        5089 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC62, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5089)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5089)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(195..1052)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1053..1157)
                     /label=AmpR promoter
     CDS             1272..1298
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1302..1328
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1332..1358
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3050..3943
                     /gene="HIS1"
                     /label=HIS1
                     /note="ATP phosphoribosyltransferase from Candida albicans 
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(4323..4356)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(4522..5089,1..21))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"