重组人AGT蛋白

人

人胚胎肾细胞（HEK293）

A DNA sequence encoding the human angiotensinogen (NP_000020.1) (Met 1-Ala 485) was fused with a polyhistidine tag at the C-terminus.

> 98 % as determined by SDS-PAGE

The recombinant human angiotensinogen comprises 463 amino acids and has a calculated molecular mass of 51.2 kDa. As a result of glycosylation, the apparent molecular mass of rh angiotensinogen is approximately 55-60 kDa in SDS-PAGE under reducing conditions.

AGT; ANGIOTENSINOGEN; PRE-ANGIOTENSINOGEN; ALPHA-1 ANTIPROTEINASE; ANHU; ANTITRYPSIN; ANGIOTENSIN LL; SERPINA8; ANGI; SERINE (OR CYSTEINE) PROTEINASE INHIBITOR; FLJ97926; ANGII; AI265500; ANGIOTENSIN II; FLJ92595; ANGIOTENSIN I; AOGEN

Angiotensinogen (Serpin peptidase inhibitor, clade A, member 8)

Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.