- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IF, FC, IP
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
GCLM antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
GCLM Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human skeletal muscle tissue. Positive FC detected in HepG2 cells. Positive WB detected in A431 cells, K-562 cells, L02 cells, MCF7 cells. Positive IP detected in A431 cells. Observed molecular weight by Western-blot: 31 kDa
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抗体应用
ELISA, WB, IHC, IF, FC, IP
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应用推荐
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
Gamma ECS regulatory subunit, GCLM, GCS light chain, GLCLR
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制备方法
This antibody was obtained by immunization of GCLM recombinant protein (Accession Number: NM_002061). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesisand is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
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参考文献
- Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.
- Gunjima K, Tomiyama R, Takakura K. 3,4-dihydroxybenzalacetone protects against Parkinson's disease-related neurotoxin 6-OHDA through Akt/Nrf2/glutathione pathway. Journal of cellular biochemistry. 115(1):151-60. 2014.
- Ma Q, Zhao J, Cao W, Liu J, Cui S. Estradiol decreases taurine level by reducing cysteine sulfinic acid decarboxylase via the estrogen receptor-α in female mice liver. American journal of physiology. Gastrointestinal and liver physiology. 308(4):G277-86. 2015.
- Snyder NW, Golin-Bisello F, Gao Y, Blair IA, Freeman BA, Wendell SG. 15-Oxoeicosatetraenoic acid is a 15-hydroxyprostaglandin dehydrogenase-derived electrophilic mediator of inflammatory signaling pathways. Chemico-biological interactions. 234:144-53. 2015.
- Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.
- Ravuri C, Svineng G, Huseby NE. Differential regulation of γ-glutamyltransferase and glutamate cysteine ligase expression after mitochondrial uncoupling: γ-glutamyltransferase is regulated in an Nrf2- and NFκB-independent manner. Free radical research. 47(5):394-403. 2013.
- Yuan XH, Fan YY, Yang CR. Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells. The Journal of steroid biochemistry and molecular biology. 155(Pt A):104-11. 2016.