重组人MGMT蛋白

人

大肠杆菌

A DNA sequence encoding the Human MGMT Met1-Asn207 is expressed with a 6His tag at the N-terminus.

>95% as determined by SDS-PAGE.

O-6-methylguanine-DNA methyltransferase(MGMT)

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Supplied as a 0.2 μm filtered solution of 20mMTrisHCl,1mM EDTA, pH 8.0 .

<1.0 EU per μg protein as determined by the LAL method.

Store at <-20°C, minimize freeze-thaw cycles.