The pESC-URA is a yeast episomal vector with a URA3 marker, for galactose-regulated expression and tagging of up to two genes.
| 载体基本信息 | |||
| 载体名称 | pESC-URA | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6631 bp | 载体类型 | Yeast Plasmids |
| 载体来源 | Agilent Technologies | 拷贝数 | High copy number |
pESC-URA 载体载体图谱
pESC-URA 载体载体序列
LOCUS 40924_17616 6631 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pESC-URA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6631)
AUTHORS Lu Q.
TITLE pESC-URA
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6631)
AUTHORS Zheng C-F.
TITLE Direct Submission
JOURNAL Submitted (06-MAY-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 3 (bases 1 to 6631)
AUTHORS Post K.
TITLE Direct Submission
JOURNAL Submitted (17-NOV-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 4 (bases 1 to 6631)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 6631)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(17-NOV-1999) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On Nov 17, 1999 this sequence version replaced AF063585.1.
FEATURES Location/Qualifiers
source 1..6631
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 196..416
/label=URA3 promoter
CDS 417..1217
/codon_start=1
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin complement(1351..1806)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
terminator complement(1885..2050)
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
CDS complement(2198..2221)
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
promoter 2268..2932
/label=GAL1,10 promoter
/note="divergent inducible promoter, regulated by Gal4"
promoter 2943..2961
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2979..3008
/codon_start=1
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
terminator 3038..3227
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(3470..4058)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4232..5089)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5090..5194)
/label=AmpR promoter
rep_origin complement(5221..6563)
/direction=LEFT
/label=2u ori
/note="yeast 2u plasmid origin of replication"
我们不提供该载体，载体信息仅供查询和参考。