载体基本信息 | |||
载体名称 | pAO815 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7709 bp | 载体类型 | Yeast Plasmids |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAO815 载体载体图谱
pAO815 载体载体序列
LOCUS Exported 7709 bp ds-DNA circular SYN 05-2-2013 DEFINITION Pichia pastoris vector for creating multimers to drive high levels of methanol-inducible protein expression. ACCESSION . VERSION . KEYWORDS pAO815 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7709) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission COMMENT The gene of interest can be inserted at the unique EcoRI site. Multimers of the resulting BglII-BamHI expression cassette can be inserted at the BamHI site. FEATURES Location/Qualifiers source 1..7709 /organism="synthetic DNA construct" /lab_host="Pichia pastoris" /mol_type="other DNA" promoter 2..940 /gene="Pichia pastoris AOX1" /note="AOX1 promoter" /note="inducible promoter, regulated by methanol" terminator 1018..1264 /gene="Pichia pastoris AOX1" /note="AOX1 terminator" /note="transcription terminator for AOX1" CDS complement(1665..4199) /codon_start=1 /gene="Pichia pastoris HIS4" /locus_tag=" " /product="multifunctional enzyme, required for histidine biosynthesis" /note="PpHIS4" /note="auxotrophic marker for Pichia pastoris" /protein_id=" " /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI" misc_feature 4554..5310 /note="AOX1 3' fragment" /note="region downstream of Pichia pastoris AOX1 gene" rep_origin complement(5780..6368) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6539..7399) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7400..7504) /gene="bla" /note="AmpR promoter"
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