载体基本信息 | |||
载体名称 | pAbAi | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4894 bp | 载体类型 | Yeast Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
pAbAi 载体载体图谱
pAbAi 载体载体序列
LOCUS pAbAi. 4894 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast integrating vector for cloning a DNA bait sequence to be used in a one-hybrid screen. ACCESSION . VERSION . KEYWORDS pAbAi. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4894) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 4894) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Linearize with BstBI or BbsI to integrate at the URA3 locus. FEATURES Location/Qualifiers source 1..4894 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..45 /label=MCS /note="MCS" /note="multiple cloning site" CDS 185..1387 /label=AurR /note="aureobasidin A-resistant mutant of S. cerevisiae inositol phosphorylceramide synthase (Aeed et al., 2009)" primer_bind complement(1645..1661) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1669..1685) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1693..1723) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1738..1759) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2047..2635) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2809..3666) /label=AmpR /note="beta-lactamase" promoter complement(3667..3771) /label=AmpR promoter CDS complement(3878..4678) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(4679..4894) /label=URA3 promoter
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