pAbAi 载体 (V010329#) COA报告 测序报告

载体基本信息
载体名称 pAbAi 载体抗性 Ampicillin
载体长度 4894 bp 载体类型 Yeast Plasmids
载体来源 Clontech 拷贝数 High copy number

pAbAi 载体载体图谱

pAbAi4894 bp6001200180024003000360042004800MCSAurRM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterURA3URA3 promoter

pAbAi 载体载体序列

LOCUS       pAbAi.        4894 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast integrating vector for cloning a DNA bait sequence to be used 
            in a one-hybrid screen.
ACCESSION   .
VERSION     .
KEYWORDS    pAbAi.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize with BstBI or BbsI to integrate at the URA3 locus.
FEATURES             Location/Qualifiers
     source          1..4894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..45
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             185..1387
                     /label=AurR
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1669..1685)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1693..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1738..1759)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2809..3666)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3667..3771)
                     /label=AmpR promoter
     CDS             complement(3878..4678)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4679..4894)
                     /label=URA3 promoter

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