载体基本信息 | |||
载体名称 | pCMV-VSV-G | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6507 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Yee JK, Miyanohara A, LaPorte P, Bouic K, Burns JC, Friedmann T. | 拷贝数 | High copy number |
pCMV-VSV-G 载体载体图谱
pCMV-VSV-G 载体载体序列
LOCUS Exported 6507 bp ds-DNA circular SYN 29-1-2014 DEFINITION Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G. ACCESSION . VERSION . KEYWORDS pCMV-VSV-G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6507) AUTHORS Yee JK, Miyanohara A, LaPorte P, Bouic K, Burns JC, Friedmann T. TITLE A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes. JOURNAL Proc. Natl. Acad. Sci. U.S.A. 1994;91:9564-8. PUBMED 7937806 REFERENCE 2 (bases 1 to 6507) AUTHORS . TITLE Direct Submission COMMENT The sequence from GenBank accession number AJ318514 was edited to match data associated with Addgene plasmid number 8454. FEATURES Location/Qualifiers source 1..6507 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 83..462 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 463..666 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" intron 778..1350 /note="beta-globin intron" /note="intron from rabbit beta-globin gene" CDS 1436..2971 /codon_start=1 /gene=" " /product="vesicular stomatitis virus G glycoprotein" /note="VSV-G" /note="Indiana strain" /protein_id=" " /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK" polyA_site 3263..3318 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" rep_origin complement(4078..4666) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4837..5697) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5698..5802) /gene="bla" /note="AmpR promoter" rep_origin complement(5828..6283) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6451..6469 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase"
我们不提供该载体,载体信息仅供查询和参考。