载体基本信息 | |||
载体名称 | pQE-30 Xa | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3509 bp | 载体类型 | Qiagen Vectors |
载体来源 | Qiagen | 拷贝数 | High copy number |
pQE-30 Xa 载体载体图谱
pQE-30 Xa 载体载体序列
LOCUS pQE-30_Xa. 3509 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing N-terminally 6xHis-tagged proteins with a Factor Xa cleavage site. ACCESSION . VERSION . KEYWORDS pQE-30 Xa. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3509) AUTHORS Qiagen TITLE Direct Submission REFERENCE 2 (bases 1 to 3509) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Clone at StuI to insert a coding sequence just after the Factor Xa cleavage site. FEATURES Location/Qualifiers source 1..3509 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" CDS 115..117 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 127..144 /label=6xHis /note="6xHis affinity tag" CDS 166..177 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" misc_feature 240..250 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" terminator 256..350 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 394..1050 /label=CmR /note="chloramphenicol acetyltransferase" terminator 1118..1204 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1359..1499 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(1685..2273) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2447..3304) /label=AmpR /note="beta-lactamase" promoter complement(3305..3409) /label=AmpR promoter
我们不提供该载体,载体信息仅供查询和参考。