pEarleyGate 204 载体 (V010858#)

载体基本信息
载体名称 pEarleyGate 204 载体抗性 Chloramphenicol
载体长度 11780 bp 载体类型 Plant Vectors
载体来源 Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. 拷贝数 High copy number

pEarleyGate 204 载体载体图谱

pEarleyGate 20411780 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000105001100011500LB T-DNA repeatMAS terminatorBlpRMAS promoterCaMV 35S promoterTMV OmegaATGAcV5 tagattR1lac UV5 promoterCmRccdBattR2OCS terminatorRB T-DNA repeatpVS1 StaApVS1 RepApVS1 oriVbomoriKanR

pEarleyGate 204 载体载体序列

LOCUS       pEarleyGate_204.       11780 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
            AcV5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    pEarleyGate 204.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "45"; pages: "616-29"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The sequence was corrected by inserting a G at position 7888.
FEATURES             Location/Qualifiers
     source          1..11780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2737..2739
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2740..2766
                     /label=AcV5 tag
                     /note="epitope tag from the baculovirus Autographa
                     californica GP64 envelope fusion protein"
     protein_bind    2770..2889
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2919..2949
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3003..3659
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             4004..4306
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4350..4474)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4551..5258
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    5502..5526
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6826..7452
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7884..8954
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9023..9217
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9561..9701
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9887..10475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10565..11356)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"

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