载体基本信息 | |||
载体名称 | pGEX-2T | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4948 bp | 载体类型 | pGEX Vectors (GE Healthcare) |
载体来源 | GE Healthcare | 拷贝数 | High copy number |
pGEX-2T 载体载体图谱
pGEX-2T 载体载体序列
LOCUS pGEX-2T. 4948 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins with a thrombin site. ACCESSION . VERSION . KEYWORDS pGEX-2T. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4948) AUTHORS GE Healthcare TITLE Direct Submission REFERENCE 2 (bases 1 to 4948) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4948 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 183..211 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 219..235 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 258..911 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 918..935 /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 950..960 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" promoter 1251..1355 /label=AmpR promoter CDS 1356..2213 /label=AmpR /note="beta-lactamase" rep_origin 2387..2975 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 3219..3296 /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 3297..4376 /label=lacI /note="lac repressor" protein_bind 4392..4413 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4428..4458 /label=lac promoter /note="promoter for the E. coli lac operon" CDS 4502..4675 /label=lacZ-alpha /note="LacZ-alpha fragment of beta-galactosidase"
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