- 载体名称:
- pGEX-1λT
- 载体抗性:
- Ampicillin
- 载体长度:
- 4947 bp
- 载体类型:
- pGEX Vectors (GE Healthcare)
- 复制子:
- ori
- 载体来源:
- GE Healthcare
- 拷贝数:
- High copy number
pGEX-1λT 载体载体图谱
pGEX-1λT 载体载体序列
LOCUS pGEX-1_lambda_T. 4947 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins. The frame of the EcoRI site matches that of lambda gt11. ACCESSION . VERSION . KEYWORDS pGEX-1_lambda_T. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4947) AUTHORS GE Healthcare TITLE Direct Submission REFERENCE 2 (bases 1 to 4947) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4947 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 183..211 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 219..235 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 258..911 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 918..935 /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 949..959 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" promoter 1250..1354 /label=AmpR promoter CDS 1355..2212 /label=AmpR /note="beta-lactamase" rep_origin 2386..2974 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 3218..3295 /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 3296..4375 /label=lacI /note="lac repressor" protein_bind 4391..4412 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4427..4457 /label=lac promoter /note="promoter for the E. coli lac operon" CDS 4501..4674 /label=lacZ-alpha /note="LacZ-alpha fragment of beta-galactosidase"
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