载体基本信息 | |||
载体名称 | pET-23(+) | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3592 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-23(+) 载体载体图谱
pET-23(+) 载体载体序列
LOCUS pET-23(+). 3592 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expression of genes with bacterial translation signals. ACCESSION . VERSION . KEYWORDS pET-23(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3592) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3592) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3592 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" misc_feature 158..203 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(227..245) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 754..942 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 1047..1189 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(1375..1963) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2137..2994) /label=AmpR /note="beta-lactamase" promoter complement(2995..3099) /label=AmpR promoter rep_origin complement(3126..3581) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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