载体基本信息 | |||
载体名称 | pET-20b(+) | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3716 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-20b(+) 载体载体图谱
pET-20b(+) 载体载体序列
LOCUS Exported 3716 bp ds-DNA circular SYN 01-12-2013 DEFINITION Bacterial vector that encodes a signal sequence for expression of proteins in the periplasm. ACCESSION . VERSION . KEYWORDS pET-20b(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3716) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..3716 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 26..73 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 158..225 /note="MCS" /note="multiple cloning site" CDS complement(224..289) /codon_start=1 /gene="pelB (fragment)" /product="leader peptide for secretion" /note="pelB signal sequence" /translation="MKYLLPTAAAGLLLLAAQPAMA" RBS 298..302 /note="ribosome binding site" promoter complement(351..369) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 878..1069 /codon_start=1 /gene="rop" /product="Rop protein" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin complement(1499..2087) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2258..3118) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3119..3223) /gene="bla" /note="AmpR promoter" rep_origin complement(3250..3705) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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