载体基本信息 | |||
载体名称 | pET-16b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5711 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-16b 载体载体图谱
pET-16b 载体载体序列
LOCUS pET-16b. 5711 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for inducible expression of N-terminally 10xHis-tagged proteins with a Factor Xa site. ACCESSION . VERSION . KEYWORDS pET-16b. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5711) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5711) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5711 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..38 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" terminator complement(213..260) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 319..335 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(336..347) /label=Factor Xa site /note="Factor Xa recognition and cleavage site" CDS complement(363..389) /label=9xHis /note="9xHis affinity tag" CDS complement(393..395) /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" RBS complement(402..424) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(439..463) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(464..482) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 791..868 /label=lacI promoter CDS 869..1948 /label=lacI /note="lac repressor" protein_bind 1964..1985 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3263..3451 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3556..3698 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3884..4472) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4646..5503) /label=AmpR /note="beta-lactamase" promoter complement(5504..5608) /label=AmpR promoter
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