载体基本信息 | |||
载体名称 | pET-14b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4671 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-14b 载体载体图谱
pET-14b 载体载体序列
LOCUS Exported 4671 bp ds-DNA circular SYN 01-12-2013 DEFINITION Bacterial vector for expression of N-terminally 6xHis-tagged proteins. ACCESSION . VERSION . KEYWORDS pET-14b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4671) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..4671 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 404..451 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 510..526 /note="MCS" /note="multiple cloning site" CDS complement(527..544) /codon_start=1 /product="thrombin recognition and cleavage site" /note="thrombin site" /translation="LVPRGS" CDS complement(554..571) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS complement(581..583) /codon_start=1 /product="start codon" /note="ATG" /translation="M" RBS 591..595 /note="ribosome binding site" promoter complement(644..662) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 2223..2414 /codon_start=1 /gene="rop" /product="Rop protein" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin complement(2844..3432) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3603..4463) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4464..4568) /gene="bla" /note="AmpR promoter"
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