载体基本信息 | |||
载体名称 | pEF1α-IRES | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6690 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Clontech | 拷贝数 | High copy number |
pEF1α-IRES 载体载体图谱
pEF1α-IRES 载体载体序列
LOCUS 40924_16910 6690 bp DNA circular SYN 01-JAN-1980 DEFINITION IRES-containing vector for expressing two genes in mammalian cells from the same bicistronic transcript. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6690) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 6690) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6690 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 154..1335 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 1475..1607 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1652..1670 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 1670..1692 /label=MCS A /note="MCS A" /note="multiple cloning site" misc_feature 1737..2323 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2332..2355 /label=MCS B /note="MCS B" /note="multiple cloning site" polyA_signal complement(2394..2515) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2701..3156 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3273..3630 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3681..4472 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 4539..4587 /label=poly(A) signal /note="synthetic polyadenylation signal" promoter 4893..4997 /label=AmpR promoter CDS 4998..5855 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6029..6617 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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