pcDNA6/myc-His C 载体 (V011290#)

载体基本信息
载体名称 pcDNA6/myc-His C 载体抗性 Ampicillin
载体长度 5122 bp 载体类型 Mammalian Expression Vectors
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number

pcDNA6/myc-His C 载体载体图谱

pcDNA6/myc-His C5122 bp6001200180024003000360042004800CMV enhancerCMV promoterT7 promoterMCS6xHisbGH poly(A) signalf1 oriSV40 promoterEM7 promoterBSDSV40 poly(A) signaloriAmpRAmpR promoter

pcDNA6/myc-His C 载体载体序列

LOCUS       Exported                5122 bp ds-DNA    circular SYN 28-11-2013
DEFINITION  Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged 
            proteins. For other reading frames, use pcDNA(TM)6/myc-His A or 
            pcDNA(TM)6/myc-His B.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA6 myc-His C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    902..995
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             993..1022
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             1038..1055
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     polyA_signal    1084..1308
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1354..1782
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1796..2125
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1976..2111
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        2173..2220
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             2239..2637
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2795..2916
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3367..3955)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4126..4986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4987..5091)
                     /gene="bla"
                     /note="AmpR promoter"

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