载体基本信息 | |||
载体名称 | pcDNA6/myc-His C | 载体抗性 | Ampicillin |
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载体长度 | 5122 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pcDNA6/myc-His C 载体载体图谱
pcDNA6/myc-His C 载体载体序列
LOCUS Exported 5122 bp ds-DNA circular SYN 28-11-2013 DEFINITION Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. For other reading frames, use pcDNA(TM)6/myc-His A or pcDNA(TM)6/myc-His B. ACCESSION . VERSION . KEYWORDS pcDNA6 myc-His C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5122) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission COMMENT The cloned gene must include a Kozak sequence and start codon. FEATURES Location/Qualifiers source 1..5122 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 902..995 /note="MCS" /note="multiple cloning site" CDS 993..1022 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" CDS 1038..1055 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" polyA_signal 1084..1308 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 1354..1782 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1796..2125 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 1976..2111 /note="SV40 ori" /note="SV40 origin of replication" promoter 2173..2220 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 2239..2637 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /note="BSD" /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 2795..2916 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3367..3955) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4126..4986) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4987..5091) /gene="bla" /note="AmpR promoter"
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