载体基本信息 | |||
载体名称 | p3xFLAG-CMV-10 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6299 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Sigma-Aldrich | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number | 5'引物 | M13 fwd |
3'引物 | M13 rev |
p3xFLAG-CMV-10 载体载体图谱
p3xFLAG-CMV-10 载体载体序列
LOCUS p3xFLAG-CMV-10. 6299 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector with a neomycin resistance marker for expression of N-terminally 3xFLAG-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS p3xFLAG-CMV-10. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6299) AUTHORS Sigma-Aldrich TITLE Direct Submission REFERENCE 2 (bases 1 to 6299) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6299 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 141..157 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 318..697 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 928..930 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 931..996 /label=3xFLAG /note="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" misc_feature 994..1060 /label=MCS /note="MCS" /note="multiple cloning site" polyA_signal 1058..1680 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" promoter 1722..2019 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2073..2864 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 3519..3653 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(3659..3677) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3691..3707) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3715..3731) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3739..3769) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3784..3805) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4093..4681) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4855..5712) /label=AmpR /note="beta-lactamase" promoter complement(5713..5817) /label=AmpR promoter rep_origin 5844..6299 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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