载体基本信息 | |||
载体名称 | pSMART LCAmp | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2025 bp | 载体类型 | Lucigen Vectors |
载体来源 | Lucigen | 拷贝数 | High copy number |
pSMART LCAmp 载体载体图谱
pSMART LCAmp 载体载体序列
LOCUS 40924_40737 2025 bp DNA circular SYN 01-JAN-1980 DEFINITION Low-copy number vector with an ampicillin resistance marker for efficient blunt cloning of unstable sequences. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2025) AUTHORS Lucigen TITLE Direct Submission REFERENCE 2 (bases 1 to 2025) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Linearize at HincII to make blunt cloning vector. FEATURES Location/Qualifiers source 1..2025 /mol_type="other DNA" /organism="synthetic DNA construct" terminator 65..96 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 97..199 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 200..1057 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" CDS 1061..1249 /codon_start=1 /label=rop /note="Rop protein, which maintains plasmids at low copy number" /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" terminator 1276..1303 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1315..1902) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(1924..1953) /label=T3Te terminator /note="phage T3 early transcription terminator"
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