pSMART HCAmp 载体图谱和序列

载体基本信息
载体名称	pSMART HCAmp	载体抗性	Ampicillin
载体长度	1833 bp	载体类型	Lucigen Vectors
载体来源	Lucigen	拷贝数	High copy number

pSMART HCAmp 载体载体图谱

pSMART HCAmp 载体载体序列

Copy Sequence

下载GeneBank文件(.gb)

LOCUS       40924_40727        1833 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy number vector with an ampicillin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      65..96
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        97..199
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             200..1057
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1084..1111
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1123..1710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1732..1761)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"

我们不提供该载体，载体信息仅供查询和参考。