载体基本信息 | |||
载体名称 | pAviTag C-His Kan | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 2359 bp | 载体类型 | Lucigen Vectors |
载体来源 | Lucigen | 拷贝数 | High copy number |
pAviTag C-His Kan 载体载体图谱
pAviTag C-His Kan 载体载体序列
LOCUS Exported 2359 bp ds-DNA circular SYN 08-1-2015 DEFINITION Vector for Expresso(R) cloning to add C-terminal biotinylation and 6xHis tags, with rhamnose-inducible expression. ACCESSION . VERSION . KEYWORDS pAviTag C-His Kan SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2359) AUTHORS Lucigen TITLE Direct Submission FEATURES Location/Qualifiers source 1..2359 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 1..119 /note="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" RBS 153..175 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 183..185 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 225..269 /codon_start=1 /product="peptide tag that allows for enzymatic biotinylation" /note="AviTag(TM)" /translation="GLNDIFEAQKIEWHE" CDS 270..287 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" terminator 319..366 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 464..493 /note="T3Te terminator" /note="phage T3 early transcription terminator" rep_origin 515..1102 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 1114..1141 /note="T7Te terminator" /note="phage T7 early transcription terminator" CDS complement(1165..1356) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(1357..2172) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(2173..2263) /note="cat promoter" /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator 2264..2295 /note="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator"
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