载体基本信息 | |||
载体名称 | pFN26A (BIND) hRluc-neo Flexi Vector | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7070 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pFN26A (BIND) hRluc-neo Flexi Vector 载体载体图谱
pFN26A (BIND) hRluc-neo Flexi Vector 载体载体序列
LOCUS pFN26A_(BIND)_hR 7070 bp DNA circular SYN 01-JAN-1980 DEFINITION Flexi(R) Vector encoding Renilla luciferase fused to a neomycin resistance gene. This vector is useful for testing transcriptional activation domains. ACCESSION . VERSION . KEYWORDS pFN26A (BIND) hRluc-neo Flexi Vector. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7070) AUTHORS Promega TITLE Direct Submission REFERENCE 2 (bases 1 to 7070) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT The protein-coding region of interest can be subcloned between the SgfI (AsiSI) and PmeI sites. FEATURES Location/Qualifiers source 1..7070 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 139..517 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 518..721 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 857..989 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1033..1051 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1083..1523 /label=GAL4 DNA binding domain /note="DNA binding domain of the GAL4 transcriptional activator" CDS 1527..1553 /codon_start=1 /product="peptide derived from a human Ig alpha chain" /label=peptide derived from a human Ig alpha chain /note="IgA linker" /translation="PSTPPTPSP" CDS 1585..1917 /label=barnase /note="ribonuclease from Bacillus amyloliquefaciens" misc_feature 1930..1985 /label=MCS /note="pUC18/19 multiple cloning site" polyA_signal complement(2090..2211) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 2456..2813 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2864..3796 /label=hRluc /note="Renilla luciferase" CDS 3848..4636 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 4703..4751 /label=poly(A) signal /note="synthetic polyadenylation signal" promoter 4907..5011 /label=AmpR promoter CDS 5012..5869 /label=AmpR /note="beta-lactamase" rep_origin complement(6030..6618) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 6734..7017 /label=cer region /note="ColE1-derived recombination site that helps to maintain plasmids as monomers"
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