- 载体名称:
- pCMV-Red Firefly Luc
- 载体抗性:
- Ampicillin
- 载体长度:
- 6575 bp
- 载体类型:
- Luciferase Vectors
- 复制子:
- ori
- 载体来源:
- Thermo Scientific (Pierce)
- 拷贝数:
- High copy number
- 启动子:
- SV40
pCMV-Red Firefly Luc 载体载体图谱
pCMV-Red Firefly Luc 载体载体序列
LOCUS 40924_11740 6575 bp DNA circular SYN 01-JAN-1980
DEFINITION Control vector for constitutive high-level expression of
intracellular red firefly luciferase under control of the CMV
promoter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6575)
AUTHORS Thermo Scientific (Pierce)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6575)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6575
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 64..367
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..571
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 616..634
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 647..2290
/codon_start=1
/label=Red Firefly Luciferase
/note="intracellular red firefly luciferase"
/translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
polyA_signal 2319..2430
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 2553..2882
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 2930..2977
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2996..3592
/codon_start=1
/label=PuroR
/note="puromycin N-acetyltransferase"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 3725..3858
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(3902..4759)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5023..5611
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5881..5897
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6353..6369
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 6484..6575
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
我们不提供该载体，载体信息仅供查询和参考。