载体基本信息 | |||
载体名称 | pCMV-GLuc 2 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5802 bp | 载体类型 | Luciferase Vectors |
载体来源 | New England Biolabs | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pCMV-GLuc 2 载体载体图谱
pCMV-GLuc 2 载体载体序列
LOCUS Exported 5802 bp ds-DNA circular SYN 03-8-2013 DEFINITION Control vector with a strong constitutive CMV promoter for expressing secreted Gaussia luciferase in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-GLuc 2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5802) AUTHORS New England Biolabs TITLE Direct Submission FEATURES Location/Qualifiers source 1..5802 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_signal 914..923 /note="Kozak sequence" CDS 920..1477 /codon_start=1 /product="secreted Gaussia luciferase" /note="hGLuc" /note="human codon-optimized" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ RCATFASKIQGQVDKIKGAGGD" polyA_signal 1486..1534 /note="synthetic polyadenylation signal" rep_origin 1668..2096 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2110..2440 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2291..2426 /note="SV40 ori" /note="SV40 origin of replication" CDS 2507..3301 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3475..3596 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(4047..4635) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4806..5666) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5667..5771) /gene="bla" /note="AmpR promoter"
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