载体基本信息 | |||
载体名称 | pAcGP67-A | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 9761 bp | 载体类型 | Insect Cell Vectors |
载体来源 | BD Biosciences | 拷贝数 | High copy number |
pAcGP67-A 载体载体图谱
pAcGP67-A 载体载体序列
LOCUS pAcGP67-A. 9761 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer vector for secreting expressed proteins using the GP64 signal sequence. For other reading frames, use pAcGP67-B or pAcGP67-C. ACCESSION . VERSION . KEYWORDS pAcGP67-A. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9761) AUTHORS BD Biosciences TITLE Direct Submission REFERENCE 2 (bases 1 to 9761) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9761 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 76..579 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2662..3291 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3170..3997 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 4001..4092 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" sig_peptide 4189..4248 /label=GP64 signal sequence /note="signal sequence from the baculovirus envelope glycoprotein GP64" misc_feature 4251..4314 /label=MCS /note="MCS" /note="multiple cloning site" misc_recomb 4776..6231 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" primer_bind complement(7142..7158) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7166..7182) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7190..7220) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7235..7256) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7544..8132) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8306..9163) /label=AmpR /note="beta-lactamase" promoter complement(9164..9268) /label=AmpR promoter primer_bind 9742..9758 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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