下载GeneBank文件(.gb)

LOCUS       pAcG3X.        8534 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing N-terminally GST-tagged 
            proteins with a Factor Xa cleavage site, for use with the BD 
            BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcG3X.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2869..2880
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    2882..2897
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     2927..4284
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(5428..5444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5657..6112
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6394..6498
                     /label=AmpR promoter
     CDS             6499..7356
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7530..8118
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8406..8427
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8442..8472
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8480..8496
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8504..8520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"