载体基本信息 | |||
载体名称 | pAcG2T | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 8530 bp | 载体类型 | Insect Cell Vectors |
载体来源 | BD Biosciences | 拷贝数 | High copy number |
pAcG2T 载体载体图谱
pAcG2T 载体载体序列
LOCUS pAcG2T. 8530 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer vector for expressing N-terminally GST-tagged proteins with a thrombin cleavage site, for use with the BD BaculoGold(TM) system. ACCESSION . VERSION . KEYWORDS pAcG2T. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8530) AUTHORS BD Biosciences TITLE Direct Submission REFERENCE 2 (bases 1 to 8530) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8530 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 761..1390 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 1269..2096 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 2100..2191 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 2206..2859 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 2866..2883 /label=thrombin site /note="thrombin recognition and cleavage site" misc_recomb 2923..4280 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" primer_bind complement(5424..5440) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5653..6108 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6390..6494 /label=AmpR promoter CDS 6495..7352 /label=AmpR /note="beta-lactamase" rep_origin 7526..8114 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8402..8423 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 8438..8468 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8476..8492 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8500..8516 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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