pAcG2T 载体 (V011689#)

载体基本信息
载体名称 pAcG2T 载体抗性 Ampicillin
载体长度 8530 bp 载体类型 Insect Cell Vectors
载体来源 BD Biosciences 拷贝数 High copy number

pAcG2T 载体载体图谱

pAcG2T8530 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400LEF-2polyhedrin promoterGSTthrombin sitecontains part of ORF1629M13 fwdf1 oriAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

pAcG2T 载体载体序列

LOCUS       pAcG2T.        8530 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing N-terminally GST-tagged 
            proteins with a thrombin cleavage site, for use with the BD 
            BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcG2T.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2866..2883
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_recomb     2923..4280
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(5424..5440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5653..6108
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6390..6494
                     /label=AmpR promoter
     CDS             6495..7352
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7526..8114
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8402..8423
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8438..8468
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8476..8492
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8500..8516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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