载体基本信息 | |||
载体名称 | pAcAB4 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 10230 bp | 载体类型 | Insect Cell Vectors |
载体来源 | BD Biosciences | 拷贝数 | High copy number |
pAcAB4 载体载体图谱
pAcAB4 载体载体序列
LOCUS pAcAB4. 10230 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer vector for expressing up to four proteins simultaneously, for use with the BD BaculoGold(TM) system. ACCESSION . VERSION . KEYWORDS pAcAB4. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10230) AUTHORS BD Biosciences TITLE Direct Submission REFERENCE 2 (bases 1 to 10230) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..10230 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 75..578 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2661..3290 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3169..3996 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" polyA_signal complement(4000..4133) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(4462..4571) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter complement(4722..4813) /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" promoter 4838..4947 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 5000..5091 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" misc_recomb 5101..6458 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" promoter 8094..8198 /label=AmpR promoter CDS 8199..9056 /label=AmpR /note="beta-lactamase" rep_origin 9230..9818 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 10106..10127 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 10142..10172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10180..10196 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10204..10220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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