载体基本信息 | |||
载体名称 | pAc5.1/V5-His B | 载体抗性 | Ampicillin |
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载体长度 | 5379 bp | 载体类型 | Insect Cell Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAc5.1/V5-His B 载体载体图谱
pAc5.1/V5-His B 载体载体序列
LOCUS pAc5.1_V5-His_B. 5379 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His A or pAc5.1/V5-His C. ACCESSION . VERSION . KEYWORDS pAc5.1 V5-His B. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5379) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 5379) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT To create stable cell lines, co-transfect with the drug selection vector pCoHygro or pCoBlast. FEATURES Location/Qualifiers source 1..5379 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 56..2568 /label=Ac5 promoter /note="Drosophila melanogaster actin 5C promoter" misc_feature 2579..2663 /label=MCS /note="MCS" /note="multiple cloning site" CDS 2664..2705 /label=V5 tag /note="epitope tag from simian virus 5" CDS 2715..2732 /label=6xHis /note="6xHis affinity tag" polyA_signal complement(2805..2939) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3561..4149) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4323..5180) /label=AmpR /note="beta-lactamase" promoter complement(5181..5285) /label=AmpR promoter
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