载体基本信息 | |||
载体名称 | pAc5.1/V5-His A | 载体抗性 | Ampicillin |
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载体长度 | 5375 bp | 载体类型 | Insect Cell Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAc5.1/V5-His A 载体载体图谱
pAc5.1/V5-His A 载体载体序列
LOCUS pAc5.1_V5-His_A. 5375 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His B or pAc5.1/V5-His C. ACCESSION . VERSION . KEYWORDS pAc5.1 V5-His A. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5375) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 5375) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT To create stable cell lines, co-transfect with the drug selection vector pCoHygro or pCoBlast. FEATURES Location/Qualifiers source 1..5375 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 56..2568 /label=Ac5 promoter /note="Drosophila melanogaster actin 5C promoter" misc_feature 2579..2659 /label=MCS /note="MCS" /note="multiple cloning site" CDS 2660..2701 /label=V5 tag /note="epitope tag from simian virus 5" CDS 2711..2728 /label=6xHis /note="6xHis affinity tag" polyA_signal complement(2801..2935) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3557..4145) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4319..5176) /label=AmpR /note="beta-lactamase" promoter complement(5177..5281) /label=AmpR promoter
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