载体基本信息 | |||
载体名称 | pAB-GST | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 10335 bp | 载体类型 | Insect Cell Vectors |
载体来源 | AB Vector | 拷贝数 | High copy number |
pAB-GST 载体载体图谱
pAB-GST 载体载体序列
LOCUS pAB-GST. 10335 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid for high-level expression of proteins with an HRV 3C-cleavable N-terminal GST tag. ACCESSION . VERSION . KEYWORDS pAB-GST. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10335) AUTHORS AB Vector TITLE Direct Submission REFERENCE 2 (bases 1 to 10335) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..10335 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 76..579 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2662..3291 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3170..3997 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 4001..4092 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 4138..4791 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 4798..4821 /label=HRV 3C site /note="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" misc_feature 4825..4888 /label=MCS /note="MCS" /note="multiple cloning site" misc_recomb 5350..6805 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" promoter complement(7764..7794) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7809..7830) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8118..8706) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8880..9737) /label=AmpR /note="beta-lactamase" promoter complement(9738..9842) /label=AmpR promoter primer_bind 10316..10332 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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