pAB-GST 载体 (V011698#)

载体基本信息
载体名称 pAB-GST 载体抗性 Ampicillin
载体长度 10335 bp 载体类型 Insect Cell Vectors
载体来源 AB Vector 拷贝数 High copy number

pAB-GST 载体载体图谱

pAB-GST10335 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000PTPLEF-2polyhedrin promoterGSTHRV 3C siteMCScontains part of ORF1629lac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

pAB-GST 载体载体序列

LOCUS       pAB-GST.       10335 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of proteins 
            with an HRV 3C-cleavable N-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-GST.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4138..4791
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             4798..4821
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    4825..4888
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     5350..6805
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(7764..7794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7809..7830)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8118..8706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8880..9737)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9738..9842)
                     /label=AmpR promoter
     primer_bind     10316..10332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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