载体基本信息 | |||
载体名称 | pAB-bee-FH | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 9783 bp | 载体类型 | Insect Cell Vectors |
载体来源 | AB Vector | 拷贝数 | High copy number |
pAB-bee-FH 载体载体图谱
pAB-bee-FH 载体载体序列
LOCUS pAB-bee-FH. 9783 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid for high-level expression of secreted proteins with a C-terminal FLAG(R)-8xHis cassette. ACCESSION . VERSION . KEYWORDS pAB-bee-FH. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9783) AUTHORS AB Vector TITLE Direct Submission REFERENCE 2 (bases 1 to 9783) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9783 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 76..579 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2662..3291 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3170..3997 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 4001..4092 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" sig_peptide 4143..4205 /label=melittin signal sequence /note="signal sequence from honeybee melittin" misc_feature 4203..4237 /label=MCS /note="MCS" /note="multiple cloning site" CDS 4236..4259 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" CDS 4269..4292 /label=8xHis /note="8xHis affinity tag" misc_recomb 4798..6253 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" primer_bind complement(7164..7180) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7188..7204) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7212..7242) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7257..7278) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7566..8154) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8328..9185) /label=AmpR /note="beta-lactamase" promoter complement(9186..9290) /label=AmpR promoter primer_bind 9764..9780 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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