pAB-bee-FH 载体 (V011700#)

载体基本信息
载体名称 pAB-bee-FH 载体抗性 Ampicillin
载体长度 9783 bp 载体类型 Insect Cell Vectors
载体来源 AB Vector 拷贝数 High copy number

pAB-bee-FH 载体载体图谱

pAB-bee-FH9783 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600PTPLEF-2polyhedrin promotermelittin signal sequenceFLAG8xHiscontains part of ORF1629M13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

pAB-bee-FH 载体载体序列

LOCUS       pAB-bee-FH.        9783 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of secreted 
            proteins with a C-terminal FLAG(R)-8xHis cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-bee-FH.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4143..4205
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    4203..4237
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             4236..4259
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4269..4292
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_recomb     4798..6253
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7164..7180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7188..7204)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7212..7242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7257..7278)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7566..8154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8328..9185)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9186..9290)
                     /label=AmpR promoter
     primer_bind     9764..9780
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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