下载GeneBank文件(.gb)

LOCUS       pAB-bee-8xHis.        9965 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of secreted 
            proteins with N- and C-terminal 8xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-bee-8xHis.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4143..4205
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             4209..4232
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             4451..4474
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_recomb     4980..6435
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(7394..7424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7439..7460)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8510..9367)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9368..9472)
                     /label=AmpR promoter