| 载体基本信息 | |||
| 载体名称 | pCMV SPORT2 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 4474 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
| 载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
| 5'引物 | M13 fwd | 3'引物 | M13 rev |
pCMV SPORT2 载体载体图谱
pCMV SPORT2 载体载体序列
LOCUS pCMV_SPORT2. 4474 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs.
ACCESSION .
VERSION .
KEYWORDS pCMV SPORT2.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4474)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4474)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4474
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(125..259)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 664..680
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 691..709
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 727..824
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter complement(832..850)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(873..889)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1015..1218)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(1219..1522)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
protein_bind complement(1552..1568)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1576..1607)
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
rep_origin complement(2069..2657)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind complement(2735..2768)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS complement(2873..3730)
/label=AmpR
/note="beta-lactamase"
rep_origin complement(4018..4473)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。