载体基本信息 | |||
载体名称 | pcDNAII | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3013 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pcDNAII 载体载体图谱
pcDNAII 载体载体序列
LOCUS pcDNAII. 3013 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with an extensive polylinker for the cloning and expression of cDNAs. ACCESSION . VERSION . KEYWORDS pcDNAII. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3013) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 3013) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3013 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 10..122 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(134..152) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(170..186) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(194..210) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(218..248) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(263..284) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(572..1160) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(1629..2007) /label=ISS /note="immunostimulatory sequence from the AmpR gene; contains unmethylated CpG dinucleotides in the context of 5'-AACGTT-3' (Sato et al., 1996)" promoter complement(2192..2296) /label=AmpR promoter rep_origin complement(2377..2832) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 2974..2990 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2997..3013 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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