| 载体基本信息 | |||
| 载体名称 | pBluescript SK(+) | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 2958 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
| 载体来源 | Short JM, Fernandez JM, Sorge JA, Huse WD. | 拷贝数 | High copy number |
| 5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescript SK(+) 载体载体图谱
pBluescript SK(+) 载体载体序列
LOCUS 40924_6801 2958 bp DNA circular SYN 01-JAN-1980
DEFINITION Standard cloning vector (phagemid excised from lambda ZAP). The f1
(+) orientation allows rescue of sense strand ssDNA. pBluescript
KS(+) has a reversed MCS.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2958)
AUTHORS Short JM, Fernandez JM, Sorge JA, Huse WD.
TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo
excision properties
JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988)
PUBMED 2970625
REFERENCE 2 (bases 1 to 2958)
AUTHORS Stratagene
TITLE Direct Submission
REFERENCE 3 (bases 1 to 2958)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "1988"; volume: "16"; issue: "15"; pages:
"7583-7600"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..2958
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(6..461)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 603..619
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature complement(653..760)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(773..791)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(812..828)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(836..852)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(860..890)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(905..926)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1214..1802)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1976..2833)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2834..2938)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。