载体基本信息 | |||
载体名称 | pBluescript SK(+) | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2958 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | Short JM, Fernandez JM, Sorge JA, Huse WD. | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescript SK(+) 载体载体图谱
pBluescript SK(+) 载体载体序列
LOCUS 40924_6801 2958 bp DNA circular SYN 01-JAN-1980 DEFINITION Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS(+) has a reversed MCS. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2958) AUTHORS Short JM, Fernandez JM, Sorge JA, Huse WD. TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988) PUBMED 2970625 REFERENCE 2 (bases 1 to 2958) AUTHORS Stratagene TITLE Direct Submission REFERENCE 3 (bases 1 to 2958) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1988"; volume: "16"; issue: "15"; pages: "7583-7600" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..2958 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(6..461) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature complement(653..760) /label=MCS /note="pBluescript multiple cloning site" promoter complement(773..791) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(812..828) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(836..852) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(860..890) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(905..926) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1214..1802) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1976..2833) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2834..2938) /label=AmpR promoter
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