| 载体基本信息 | |||
| 载体名称 | pcDNA6.2/nLumio-DEST | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6796 bp | 载体类型 | Gateway Cloning Vectors |
| 载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pcDNA6.2/nLumio-DEST 载体载体图谱
pcDNA6.2/nLumio-DEST 载体载体序列
LOCUS pcDNA6.2_nLumio- 6796 bp DNA circular SYN 01-JAN-1980
DEFINITION Mammalian Gateway(R) destination vector for N-terminal tagging of a
protein with the Lumio(TM) tetracysteine tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA6.2 nLumio-DEST.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6796)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6796)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6796
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 912..914
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 924..941
/label=tetracysteine tag
/note="tetracysteine peptide that binds biarsenical
labeling reagents"
CDS 957..998
/label=V5 tag
/note="epitope tag from simian virus 5"
protein_bind 1011..1135
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 1160..1190
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 1244..1900
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 2224..2526
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2570..2694)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
polyA_signal 2781..2829
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3031..3459
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3473..3802
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 3850..3897
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 3916..4311
/label=BSD
/note="blasticidin S deaminase"
polyA_signal 4472..4605
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4642..4658)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4666..4682)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4690..4720)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4735..4756)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5044..5629)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5803..6660)
/label=AmpR
/note="beta-lactamase"
promoter complement(6661..6765)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。