| 载体基本信息 | |||
| 载体名称 | pAd/CMV/V5-DEST | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 36686 bp | 载体类型 | Gateway Cloning Vectors |
| 载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAd/CMV/V5-DEST 载体载体图谱
pAd/CMV/V5-DEST 载体载体序列
LOCUS pAd_CMV_V5-DEST. 36686 bp DNA circular UNA 01-JAN-1980
DEFINITION Adenoviral Gateway(R) destination vector for constitutive expression
of a C-terminally V5-tagged protein.
ACCESSION .
VERSION .
KEYWORDS pAd CMV V5-DEST.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 36686)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 36686)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..36686
/mol_type="genomic DNA"
/organism="synthetic DNA construct"
repeat_region 1..103
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
enhancer 731..1110
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1111..1314
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1359..1377
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1407..1531
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(1963..2265)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(2610..3266)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(3267..3369)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(3547..3671)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 3697..3738
/label=V5 tag
/note="epitope tag from simian virus 5"
polyA_signal 3822..3870
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS 4152..4571
/gene="IX"
/label=IX
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(4637..5971)
/gene="IVa2"
/label=IVa2
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
CDS complement(5743..9324)
/gene="POL"
/label=POL
/note="DNA polymerase from Human adenovirus C serotype 2.
Accession#: P03261"
CDS complement(9129..11132)
/gene="PTP"
/label=PTP
/note="Preterminal protein from Human adenovirus C serotype
5. Accession#: P04499"
CDS 11593..12837
/gene="L1"
/label=L1
/note="Packaging protein 3 from Human adenovirus C serotype
5. Accession#: P04496"
CDS 12861..14615
/note="Pre-hexon-linking protein IIIa from Human adenovirus
C serotype 5. Accession#: P12537"
CDS 14699..16411
/gene="L2"
/label=L2
/note="Penton protein from Human adenovirus C serotype 5.
Accession#: P12538"
CDS 16421..17014
/gene="L2"
/label=L2
/note="Pre-histone-like nucleoprotein from Human adenovirus
C serotype 2. Accession#: P68950"
CDS 17087..18190
/gene="L2"
/label=L2
/note="Core-capsid bridging protein from Human adenovirus C
serotype 5. Accession#: P24938"
CDS 18221..18460
/note="Pre-core protein X from Human adenovirus C serotype
5. Accession#: Q2KS10"
CDS 18546..19295
/gene="L3"
/label=L3
/note="Pre-protein VI from Human adenovirus C serotype 5.
Accession#: P24937"
CDS 19385..22240
/gene="L3"
/label=L3
/note="Hexon protein from Human adenovirus C serotype 5.
Accession#: P04133"
CDS 22276..22887
/gene="L3"
/label=L3
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(22989..24575)
/gene="DBP"
/label=DBP
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 24604..27024
/gene="L4"
/label=L4
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 27717..28397
/gene="L4"
/label=L4
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS 29707..31449
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
CDS complement(31861..32742)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
CDS complement(33021..33368)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
CDS complement(33809..34192)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
repeat_region 34502..34604
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(34828..35416)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(35571..36428)
/label=AmpR
/note="beta-lactamase"
promoter complement(36429..36533)
/label=AmpR promoter
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