载体基本信息 | |||
载体名称 | pAd/BLOCK-iT-DEST | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 34864 bp | 载体类型 | Gateway Cloning Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAd/BLOCK-iT-DEST 载体载体图谱
pAd/BLOCK-iT-DEST 载体载体序列
LOCUS Exported 34864 bp ds-DNA circular SYN 18-11-2013 DEFINITION Adenoviral Gateway(R) destination vector for inserting an shRNA cassette from a U6 entry vector. ACCESSION . VERSION . KEYWORDS pAd BLOCK-iT-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 34864) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission FEATURES Location/Qualifiers source 1..34864 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" repeat_region 1..103 /note="5' ITR" /note="inverted terminal repeat of human adenovirus serotype 5" misc_signal 191..341 /note="Ad5 Psi" /note="packaging signal for adenovirus serotype 5" protein_bind 512..636 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR1" /note="recombination site for the Gateway(R) LR reaction" promoter 661..691 /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" CDS 745..1404 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 1746..2051 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(2092..2216) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" misc_feature 2234..32782 /note="adenoviral DNA" /note="sequences from human adenovirus serotype 5, lacking the E1 and E3 regions" repeat_region 32680..32782 /note="3' ITR" /note="inverted terminal repeat of human adenovirus serotype 5" rep_origin complement(33006..33594) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(33746..34606) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPA?MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(34607..34711) /gene="bla" /note="AmpR promoter"
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