| 载体基本信息 | |||
| 载体名称 | pAC149-pCR8-dCas9VP160 | 载体抗性 | Spectinomycin/Streptomycin |
|---|---|---|---|
| 载体长度 | 7442 bp | 载体类型 | CRISPR Plasmids |
| 载体来源 | Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan | 拷贝数 | High copy number |
pAC149-pCR8-dCas9VP160 载体载体图谱
pAC149-pCR8-dCas9VP160 载体载体序列
LOCUS pAC149-pCR8-dCas 7442 bp DNA circular SYN 01-JAN-1980
DEFINITION Gateway(R) entry vector with a cassette encoding catalytically
inactive dCas9 fused to the VP160 transcriptional activation domain.
ACCESSION .
VERSION .
KEYWORDS pAC149-pCR8-dCas9VP160.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7442)
AUTHORS Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan
S, Shivalila CS, Dadon DB, Jaenisch R.
TITLE Multiplexed activation of endogenous genes by CRISPR-on, an
RNA-guided transcriptional activator system.
JOURNAL Cell Res. 2013;23:1163-71.
PUBMED 23979020
REFERENCE 2 (bases 1 to 7442)
AUTHORS Jaenisch Lab / Addgene #48221
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7442)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res.";
date: "2013"; volume: "23"; pages: "1163-71"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7442
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/label=attL1
/note="recombination site for the Gateway(R) LR reaction"
CDS 689..691
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 692..718
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/note="HA"
/translation="YPYDVPDYA"
CDS 722..742
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
CDS 752..4852
/label=dCas9
/note="catalytically dead mutant of the Cas9 endonuclease
from the Streptococcus pyogenes Type II CRISPR/Cas system"
CDS 4856..4876
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
CDS 4910..5293
/label=VP160
/note="10 tandem repeats of the minimal activation domain
of herpes simplex virus VP16 (Cheng et al., 2013)"
protein_bind complement(5321..5420)
/label=attL2
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(5438..5456)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5461..5477)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5908..6696
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
rep_origin 6792..7380
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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