载体基本信息 | |||
载体名称 | pT7Blue | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2887 bp | 载体类型 | Cloning Vectors |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pT7Blue 载体载体图谱
pT7Blue 载体载体序列
LOCUS pT7Blue. 2887 bp DNA circular SYN 01-JAN-1980 DEFINITION Cloning vector with a T7 promoter, and with a modified multiple cloning site that includes an EcoRV site for blunt cloning or TA cloning. ACCESSION . VERSION . KEYWORDS pT7Blue. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2887) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 2887) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2887 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 24..42 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 45..125 /label=MCS /note="MCS" /note="multiple cloning site" primer_bind complement(126..142) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(284..739) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 766..870 /label=AmpR promoter CDS 871..1728 /label=AmpR /note="beta-lactamase" rep_origin 1902..2490 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2778..2799 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2814..2844 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2852..2868 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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