载体基本信息 | |||
载体名称 | pSP73 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2464 bp | 载体类型 | Cloning Vectors |
载体来源 | Krieg PA, Melton DA. | 拷贝数 | High copy number |
pSP73 载体载体图谱
pSP73 载体载体序列
LOCUS 40924_41032 2464 bp DNA circular SYN 01-JAN-1980 DEFINITION Cloning vector for in vitro transcription using the SP6 and T7 RNA polymerase promoters. Essentially identical to pSP72 except for the orientation of the MCS. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2464) AUTHORS Krieg PA, Melton DA. TITLE In vitro RNA synthesis with SP6 RNA polymerase. JOURNAL Meth. Enzymol. 1987;155:397-415. PUBMED 2828872 REFERENCE 2 (bases 1 to 2464) AUTHORS Promega TITLE Direct Submission REFERENCE 3 (bases 1 to 2464) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Meth. Enzymol."; date: "1987"; volume: "155"; pages: "397-415" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..2464 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 24..80 /label=MCS /note="pUC18/19 multiple cloning site" promoter complement(102..120) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(378..966) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1140..1997) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1998..2102) /label=AmpR promoter promoter 2448..2464 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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