pSP73 载体 (V012396#)

载体基本信息
载体名称 pSP73 载体抗性 Ampicillin
载体长度 2464 bp 载体类型 Cloning Vectors
载体来源 Krieg PA, Melton DA. 拷贝数 High copy number

pSP73 载体载体图谱

pSP732464 bp600120018002400MCST7 promoteroriAmpRAmpR promoterSP6 promoter

pSP73 载体载体序列

LOCUS       40924_41032        2464 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector for in vitro transcription using the SP6 and T7 RNA 
            polymerase promoters. Essentially identical to pSP72 except for the 
            orientation of the MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Krieg PA, Melton DA.
  TITLE     In vitro RNA synthesis with SP6 RNA polymerase.
  JOURNAL   Meth. Enzymol. 1987;155:397-415.
  PUBMED    2828872
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Meth. 
            Enzymol."; date: "1987"; volume: "155"; pages: "397-415"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    24..80
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(102..120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(378..966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1140..1997)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1998..2102)
                     /label=AmpR promoter
     promoter        2448..2464
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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