The pACYC177 Plasmid containing the p15A origin of replication and a kanamycin resistance gene.
载体基本信息 | |||
载体名称 | pACYC177 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3941 bp | 载体类型 | Cloning Vectors |
载体来源 | New England Biolabs | 拷贝数 | Medium copy number |
pACYC177 载体载体图谱
pACYC177 载体载体序列
LOCUS 40924_3966 3941 bp DNA circular SYN 01-JAN-1980 DEFINITION Plasmid containing the p15A origin of replication and a kanamycin resistance gene. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3941) AUTHORS New England Biolabs TITLE Direct Submission REFERENCE 2 (bases 1 to 3941) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Compatible with plasmids containing the colE1/pMB1/pBR322/pUC origin of replication. FEATURES Location/Qualifiers source 1..3941 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 783..1328 /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS 1923..2735 /codon_start=1 /label=KanR /note="aminoglycoside phosphotransferase" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter 3594..3698 /label=AmpR promoter CDS join(3699..3941,1..615) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 3883..3941 /label=ISS /note="immunostimulatory sequence from the AmpR gene; contains unmethylated CpG dinucleotides in the context of 5'-AACGTT-3' (Sato et al., 1996)"
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