载体基本信息 | |||
载体名称 | LITMUS39 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2817 bp | 载体类型 | Cloning Vectors |
载体来源 | Evans PD, Cook SN, Riggs PD, Noren CJ. | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
LITMUS39 载体载体图谱
LITMUS39 载体载体序列
LOCUS Exported 2817 bp ds-DNA circular SYN 25-11-2013 DEFINITION High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS38. ACCESSION . VERSION . KEYWORDS LITMUS39 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2817) AUTHORS Evans PD, Cook SN, Riggs PD, Noren CJ. TITLE LITMUS: multipurpose cloning vectors with a novel system for bidirectional in vitro transcription. JOURNAL BioTechniques 1995;19:130-5. PUBMED 7669286 REFERENCE 2 (bases 1 to 2817) AUTHORS New England Biolabs TITLE Direct Submission FEATURES Location/Qualifiers source 1..2817 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 38..142 /gene="bla" /note="AmpR promoter" CDS 143..1003 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(1045..1554) /direction=LEFT /note="M13 ori" /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin 1665..2253 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2350..2380 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2388..2404 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2412..2428 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 2424..2789 /codon_start=1 /gene="lacZalpha" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITPSYVIRLTSGSTACLYTPEDAFGRASEFWIHDILQEAFWR LAGAIEWAPLVKALSESYYGLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD RPSQQLRSLNGEWRFAW" promoter 2449..2466 /gene="lacZalpha" /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" misc_feature 2460..2602 /gene="lacZalpha" /note="MCS" /note="multiple cloning site" promoter complement(2597..2614) /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2618..2634) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants"
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